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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts just isn't yet readily available.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope acknowledged with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best success are...
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This information outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Target Info Cyclin E belongs to the highly conserved cyclin family members, whose customers are characterised by a spectacular periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which lead to your temporal coordination of each mitotic function. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates for the G1-S phase boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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